CPR2009 Control of Pig Reproduction VIII Physiological Roles of The Boar Ejaculate (4 abstracts)
Recent advances in boar semen cryopreservation
D. Rath 1 , R. Bathgate 2 , H. Rodriguez-Martinez 3 , J. Roca 4 & J. Strzezek 5 and D. Waberski
1Institute of Farm Animal Genetics, Friedrich Loeffler Institute, Federal Institute of Animal Health, 31535 Neustadt-Mariensee, Germany; 2Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia; 3Division of Reproduction, Faculty of Veterinary Medicine and Animal Sciences, SLU, SE-75007 Uppsala, Sweden; 4Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30071 Murcia, Spain; 5Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Poland;6Unit for Reproductive Medicine of Clinics for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, 30559 Hannover, Germany
Since 35 years ago boar semen has been frozen and used for artificial insemination (Al). However, fertility of cryopreserved porcine sperm has consistently been low as boar sperm are more sensitive to cellular stress imposed by changing osmotic balance, oxidative stress, low-temperature exposure, cryo-protectant intoxication etc. and are less able to compensate for these deficiencies at commercially applicable dosages. Additionally, differences in sperm freezability among individuals are well known. Here we review current advances on tests to screen sperm quality post-thaw, on ways of diminishing individual boar effects, on improvement of cryoprotection by novel extender components, on packaging and freezing protocols and freezing and thawing methods, and on the handling of sexed boar sperm. Major advances have been registered, which have improved cryo-survival and the capacity to process boar semen for commercial Al.
© 2009 Society for Reproduction and Fertility
Volume 18
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