CPR2013 Control of Pig Reproduction IX Gestation Elicited (4 abstracts)
Application of RNA-seq transcriptomic analysis to reproductive physiology of the pig: Insights into differential trophoblast function within the late gestation porcine placenta
Anthony K. McNeel 1 , Celine Chen 2 , Steven Schroeder 3 , Tad Sonstegard 3 & Harry Dawson 2 and Jeffrey L. Vallet
1Reproduction Research Unit, USDA*, ARS U.S. Meat Animal Research Center, Clay Center, NE 68933-0166; 2 Diet, Genomics and Immunology Laboratory, 3Bovine Functional Genomics Laboratory, USDA, ARS Beltsville Agricultural Research Center, Beltsville, MD 20705
Next generation DNA sequencing is a high throughput method of sequencing DNA samples in parallel. During the last 10 years, this technology has expanded to include sequencing and quantification of an entire transcriptome. The advantage of this method of transcriptome analysis is that it allows the investigator to detect previously unknown genes and splice variants as well as detect potential DNA polymorphisms. Application of this technology, especially when used to perform cDNA sequencing, allows for comprehensive characterization of transcriptomes between cell types, tissues or under different physiological states. In this review, we summarize high throughput transcriptome analysis, the sequencing platforms currently available, some of the software needed to handle the data generated and how to develop a picture of what the data means from a physiologist’s point of view. Lastly, we describe an example of this type of analysis applied to porcine placental trophoblast cell types.
© 2013 Context Products Ltd
Volume 19
Article tools
My recent searches
My recently viewed abstracts
Authors
Bioscientifica Proceedings
ISSN 2515-0227 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more