Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0012cpr9 | Manipulation of The Embryo | CPR1985

Culture and storage of pig embryos

Davis D. L. ,

Summary. Studies have consistently demonstrated that 4-cell pig embryos can be cultured to the blastocyst stage in a simple salt solution containing bovine serum albumin (BSA). Pig embryos appear to be detrimentally affected by lower levels of lactate and pyruvate than are mouse embryos, but in general their in-vitro requirements are similar. Results from embryos cultured between the 4-cell and blastocyst stages are consistent enough to allow the use of c...

bp0014cpr10 | Conceptus-Uterine Interactions in Pigs | CPR1993

Studies of uterine secretions and products of primary cultures of endometrial cells in pigs

Davis D. L. , Blair R. M. ,

The uterus plays a central role in the reproductive biology of mammals. Adaptation of the uterus from an oviparous to a viviparous nature required changes that involved production of a uterine environment that could support the development of the embryo and fetus. Production of a suitable environment includes the synthesis and secretion of products by the uterine endometrium. However, the uterine endometrium is not a single homogeneous unit, but rather consists of several...

bp0006rdr14 | Reproductive Manangement | REDR2006

Technologies for fixed-time artificial insemination and their influence on reproductive performance of Bos indicus cattle

Bó GA , Cutaia L , Peres LC , Pindnato D , Maraña D , Baruselli PS

The adaptation of Bos indicus cattle to tropical and subtropical environments has led to their widespread distribution around the world. Although artificial insemination (AI) is one of the best alternatives to introduce new genetics into Bos indicus herds, the peculiarity of their temperament and the tendency to show short oestrus (many of them during the night) greatly affects the effectiveness of genetic improvement programs. Therefore, the most useful alte...

bp0014cpr20 | Components of Prolificacy in Pigs | CPR1993

Uterine function in Meishan pigs

Christenson R. K. , Vallet J. L. , Leymaster K. A. , Young L. D.

The Meishan pig provides a biological model with the genetic capacity to express a high prolificacy. This prolificacy can be partially attributed to a higher ovulation rate and a higher rate of prenatal survival at a given ovulation rate throughout gestation than in European breeds. Both early embryonic survival (factors inherent to the ovum and uterus, which occur before day 25 of gestation) and uterine capacity (factors inherent to uterine limitation, which occur from 3...

bp0013cpr17 | Gamete Physiology | CPR1989

Expression and performance in transgenic pigs

Pursel V. G. , Bolt D. J. , Miller K. F. , Pinkert C. A. , Hammer R. E. , Palmiter R. D. , Brinster R. L.

Summary. Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called ‘transgenic’. The percentage of injected ova that developed into transgenic pigs varied among experiments from 0.31% to 1.73%. The percentage of transgenic pigs that expressed the fusion gene ranged from 17% to 100%.Eleven different regulatory s...

bp0005rdr26 | Genes Controlling Reproductive Performance | REDR2002

Prolificacy genes in sheep: the French genetic programmes

Mulsant P , Lecerf F , Fabre S , Bodin L , Thimonier J , Monget P , Lanneluc I , Monniaux D , Teyssier J , Elsen J-M

It has been demonstrated that variations in litter size or ovulation rate in different breeds of sheep can be associated with the segregation of several major genes. This set of natural mutants constitutes a valuable resource to determine key points in the biochemical pathways controlling the development of ovarian follicles. The French genetic programmes were devised to identify two of these genes: the Booroola (FecB) and Lacaune genes. The FecB pro...