Bioscientifica Proceedings

Activation of its genome is amongst the essential task the embryo has to undertake following fertilization of the egg. In animal and plants, this activation follows a period of transcriptional silence, which is made necessary by the requirement for an almost complete and functional reprogramming of the DNA coming from both gametes. The process by which DNA is silenced, reprogrammed and reactivated is not fully understood yet but progresses are being made, especially with the h...

In vitro culture has provided new information on the mechanisms involved in fertilization where two completely different cells fuse together. At the same time, results obtained in vitro have led to new questions. Does the follicle influence the final maturation process of the oocyte and does the oviduct regulate the normal behaviour of spermatozoa? Recent studies indicate a critical influence of both the follicular compartment and the oviduct on the norm...

Oocyte maturation, a simple and visible phenomenon, is about to be transformed into a complex and not so visible molecular cascade leading to the marking of the following generation. The study of oocyte maturation in mammals is progressively changing towards a more molecular approach. This review addresses the main challenges in the study of RNA extraction and quantification in oocytes and embryos as well as the importance of the mRNA maturation. The identification of specific...

Abstract unavailable© 1982 The authors...

Fixed time insemination is a strategy that may facilitate batch management of swine units. After briefly discussing the advantages and disadvantages of the drugs available to induce ovulation in swine and their optimal timing of administration, the reproductive performance of a scheme combining ovulation induction and a single fixed time artificial insemination will be described for gilts and sows. In gilts, this single fixed time insemination regimen includes a GnRH ago...

The development of technologies to grow and mature oocytes from the most abundant primordial follicles holds many attractions for clinical practice, animal production technology and research. However, despite much research attention, it has proved difficult to grow follicles from early stages to maturity in vitro, as relatively little is known about the biology of oogenesis. It is clear that throughout oocyte development in vivo, follicle cell support is fund...

Summary. Maternal recognition of pregnancy in sheep, cattle and goats involves physiological mechanisms that result in protection of corpora lutea from luteolysis by modification or inhibition of uterine production of luteolytic pulses of prostaglandin (PG) F-2α. Ovine, bovine and caprine luteal cells release oxytocin in a pulsatile manner during late dioestrus. Oxytocin then binds to its endometrial receptors and initiates luteolytic pulses of PGF-2&#945...

Unlike in sheep, in which immunization against androstenedione causes mild and reasonably controlled increased ovulation rate, in similar studies cattle showed highly variable responses ranging from increased ovulation rate and fertility through to anovulation/anoestrus or superovulation. As a consequence, interest in manipulation of ovulation rate through this approach has declined and is now focused on immunological manipulation of endogenous inhibin following successful stu...

Despite the great potential of sperm technologies such as sperm cryopreservation and sperm sex sorting for the improvement of different aspects of swine production, artificial insemination with fresh or stored semen is currently the only sperm technology used at a commercial scale in the pig industry. The lower reproductive performance associated with the use of these sperm technologies is the reason for such limited use. Since optimal characteristics are required for su...

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