Bioscientifica Proceedings

Proteomic analysis occupies an increasingly important place in gamete and embryo biology as an independent tool of discovery and as a means of follow-up to transcriptional profiling. Proteomics have been and will be increasingly helpful in many areas of reproductive biology, including applied science and technology development. Areas likely to be impacted most rapidly by proteomic knowledge include fertility evaluation in male farm animals, male infertility diagnostics i...

Light microscopic semen evaluation provides useful information about a given sperm sample, but due to its subjective nature has limited prognostic value for the reproductive performance of males or the outcome of assisted fertilization. Cryptic sperm abnormalities (occurring at the molecular level) are not easily detectable by light microscopy, but can be revealed by an array of biomarkers. The latter include fluorescent markers of acrosomal status, fluorochromes detecting alt...