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Although mouse embryonic stem cells have been used widely for over a decade as an important tool for introducing precise genetic modification into the genome, demonstrating the great value of this technology in a range of biomedical applications, similar technology does not exist for domestic animals. However, the development of somatic cell nuclear transfer has bypassed the need for embryonic stem cells from livestock. The production of offspring from differentiated cel...

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bp0016cpr18 | Gestation and Parturition | CPR2001

Prenatal development as a predisposing factor for perinatal losses in pigs

van der Lende T. , Knol E. F. , Leenhouwers J. I.

The pig industry is confronted with substantial losses due to piglet mortality. With 3-8% stillbirths and generally > 10% preweaning mortality, approximately one fifth of al I fetuses formed fully at the end of gestation die before weaning. Most of these losses occur in the perinatal period. Overall prenatal development (birth weight) and specific prenatal developmental and maturational processes in late gestation are predisposing factors for perinatal losses. Birth weight ...

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bp0017cpr24 | (1) | CPR2005

Germ cell transplantation in pigs - advances and applications

Dobrinski I.

Transplantation of germ cells from fertile donor mice to the testes of infertile recipient mice results in donor-derived spermatogenesis and transmission of the donor haplotype to offspring of recipient animals. In the pig, germ cells can be transplanted to a recipient testis by ultrasoundguided cannulation of the rete testis with delivery of cells by gravity flow. It is important to note that germ cell transplantation was successful between unrelated, immuno-competent p...

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bp0002rdr21 | Transgenesis | REDR1990

Basic techniques for transgenesis

Wilmut I , Clark AJ

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bp0009rdr1 | (1) | REDR1986

Control of GnRH secretion

Clarke I. J.

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bp0005rdr9 | Reproductive Technology | REDR2002

Morphological assessment of preimplantation embryo quality in cattle

Maddox-Hyttel P , Gjørret JO , Vajta G , Alexopoulos NI , Lewis I , Trounson A , Viuff D , Laurincik J , Müller M , Tveden-Nyborg P , Thomsen PD

The extensive use of embryo technologies has emphasized the need for assessing embryo quality by morphological techniques, such as transmission electron microscopy, immunocytochemistry for confocal laser scanning microscopy and fluorescence in situ hybridization. By a combination of these techniques, it has been possible to demonstrate: (i) that rRNA gene activation, as monitored by embryonic nucleolar development, is comparable in bovine embryos developed in vivo...

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bp0003rdr40 | Short Communications | REDR1994