Bioscientifica Proceedings

Embryo transfer (ET) should play a critical role in the pig industry because it allows the movement and introduction of new genetic material into a herd with minimal risk of disease transmission and reduced transportation costs. In addition, embryo movement could prevent the potential health and welfare problems associated with transporting live pigs. Although the first successful ET was reported more than 60 years ago, the commercial use of this procedure in pigs is sti...

A new method for non-surgical deep intrauterine catheterization of pigs, without sedation of the sow, is described. Insemination results obtained with this method using fresh spermatozoa demonstrate that, in comparison to conventional artificial insemination (Al) (3 × 109 spermatozoa in 80-100 ml), a 20-60-fold reduction in the number of spermatozoa inseminated and at least a 8-10-fold reduction in the dose volume can be used without affecting fertility ...

Although cryopreservation of boar semen for artificial insemination (Al) was developed 35 years ago, cryopreservation conditions and Al strategies are still considered sub-optimal. Al with excessive numbers of frozenthawed sperm (5-6 x 109 cells), still does not achieve fertility levels similar to Al using liquid semen because of reduced sperm survival. Frozenthawed (FT) spermatozoa have therefore not been the preferred option for commercial breeding programme...

Despite the great potential of sperm technologies such as sperm cryopreservation and sperm sex sorting for the improvement of different aspects of swine production, artificial insemination with fresh or stored semen is currently the only sperm technology used at a commercial scale in the pig industry. The lower reproductive performance associated with the use of these sperm technologies is the reason for such limited use. Since optimal characteristics are required for su...

During ejaculation in the boar, sperm cohorts emitted in epididymal cauda fluid are sequentially exposed and resuspended in different mixtures of accessory sex gland secretion. This paper reviews the relevance of such unevenly composed fractions of seminal plasma (SP) in vivo on sperm transport and sperm function and how this knowledge could benefit boar semen processing for artificial insemination (Al). The firstly ejaculated spermatozoa (first 10 ml of the spe...

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In all vertebrate species studied, the main central population of GnRH neurones, which produces the final messages regulating reproduction, originates outside the brain. Early during fetal life, they appear in the olfactory placode epithelium and then migrate toward the base of the telencephalon in close association with the nervus terminalis, penetrate the brain within the nervus terminalis roots, reach their final locations and eventually grow axons toward their targets. Onl...

Implantation is a critical step in the progress of pregnancy, during which the conceptus acquires a fixed position within the uterine lumen, and leads to the establishment of the placental structures. This process implies some cellular modifications of both the uterine epithelium and the trophoblast to ensure cell adhesion between the two tissues. In ruminants, the implantation process is characterized by three main steps: a long pre-attachment period lasting 2–3 weeks d...

The development of new technologies that would increase the efficiency for generation of transgenic livestock and would overcome some of the problems associated with random insertion of the transgene will greatly benefit animal agriculture. A potential alternative technology to pronuclear injection for the generation of transgenic pigs involves the isolation, culture and genetic manipulation of cell lines that can be reintroduced into the embryo for participation in the f...

Summary. In the ram, the size of the testes is related to the concentrations of FSH, LH and testosterone in the blood. A significant linear relationship is observed between testicular size and the level of FSH until a maximum after which no further increase in testis size is observed. Testicular size is also linearly related to the mean level (25 consecutive hours of sampling) of LH and testosterone in the blood, with apparently no upper limit, and to the freq...