Bioscientifica Proceedings

In mammals, growing oocytes have characteristically high levels of RNA synthesis. After the initiation of meiosis, that is germinal vesicle breakdown, this RNA synthesis ceases. Although there is limited evidence for RNA synthesis by the zygote, significant amounts of RNA synthesis do not occur until a species-specific cell stage. In pigs, significant amounts of mRNA synthesis cannot be detected before the four-cell stage. There appear to be three qualitatively different ...

The inconsistency of data from experiments designed to show nutritional effects on embryonic survival is perplexing. However, a number of experimental models have provided some insight into the mechanisms that potentially mediate interactions between nutrition, metabolic state and embryonic survival. The developing ovarian follicle provides the maturational environment for the oocyte, and differences in follicular maturation are associated with differences in the ability ...

Much effort is being made to establish relationships between the molecular events that take place in spermatozoa under fertilizing conditions and actual sperm function during fertilization. During capacitation, the process that 'primes' spermatozoa for interaction with the egg, components of the sperm's environment, notably bicarbonate, provoke various specific changes in the architecture and functioning of the sperm plasma membrane in a large number of cells. The individ...

The changing global needs for food and animal products require the development of breeding strategies for maximizing genetic improvement while maintaining genetic diversity. Genetic diversity can be conserved by using separate breeding herds; however, they may be expensive to maintain and inbreeding becomes a major concern. Alternative methods are needed to preserve valuable genetic resources in a reasonable and economic manner. Embryo cryopreservation allows indefinite s...

Fertilization of the egg, or oocyte, initiates the entire developmental process, but while the mechanism by which the spermatozoa triggers the oocyte to resume meiosis has been studied extensively, conclusions about this process are still elusive. Some workers have suggested that a molecule on the surface of the spermatozoon may interact with a receptor on the plasma membrane of the oocyte, thereby triggering the oocyte to resume meiosis. Other workers have focused on a ...

Considerable progress has been made in the in vitro production of pig embryos using improved methods for in vitro maturation (IVM) and fertilization (IVF). Despite the progress, polyspermic penetration remains a problem for in vitro-matured oocytes. Variation among boars, ejaculates and IVF protocols used in different laboratories appears to influence the incidence of polyspermy. Recent studies indicate that oviduct cells and their secretions p...

Great advancements in cryopreservation of pig embryos have been made since the last International Conference on Pig Reproduction (ICPR). In 1997, there were standard methods to cryopreserve germplasm and embryos of most livestock species, except for the pig, and development of this technology for use in the international pig industry was slow and in the early stages. Since 1997, there have been advancements in cryopreservation of pig embryos, with reports of production o...

In contrast to nuclear inheritance, cytoplasmic inheritance in mammals is derived mostly, if not exclusively, from the maternal line. Mitochondria, and their DNA molecules (mtDNA), are the genetic units of this method of inheritance. Mammalian mtDNA codes for 13 enzymes used in the mitochondrial energy-generating pathway, oxidative phosphorylation, 22 tRNAs and two rRNAs. Although all transcripts of mtDNA and their translational products remain in the mitochondria, most ...

Pigs begin behavioural preparations for birth about 1-2 days before parturition. Prepartum sows wander to select a suitable site and then construct a maternal nest. The signal that initiates this behavioural cascade probably results from fetal maturation but is unknown. However, endogenous PGF2a appears to be involved early on in an endocrine pathway that projects to the brain and can generate most of the prepartum behavioural components. This period of intense activity ...

Abstract unavailable© 2009 Society for Reproduction and Fertility...