Bioscientifica Proceedings

Light microscopic semen evaluation provides useful information about a given sperm sample, but due to its subjective nature has limited prognostic value for the reproductive performance of males or the outcome of assisted fertilization. Cryptic sperm abnormalities (occurring at the molecular level) are not easily detectable by light microscopy, but can be revealed by an array of biomarkers. The latter include fluorescent markers of acrosomal status, fluorochromes detecting alt...

Summary. Concentrations of β-endorphin were quantified in peripheral blood plasma of sheep by a radioimmunoassay that cross-reacted with β-lipotrophin. Plasma concentrations of β-endorphin increased abruptly after physical confinement, bacteraemia, and electroacupuncture treatment for induction of analgesia. In these experimental situations in which plasma concentrations of β-endorphin increased, plasma concentrations of LH often decreased....

Keywords: pig; metabolism; hypothalamus; ovary; pituitary© 1990 Journals of Reproduction & Fertility Ltd...

Sex ratios that deviate from 1:1 have been observed in response to a number of stimuli. In this review we will discuss sex ratio biasing, and the evolutionary and molecular mechanisms thought to underlie this phenomena in mammals. The role of embryo quality will be discussed in relation to sex ratio modulation and epigenetic programing of the embryo. Sex ratio skewing has been studied in many species and several factors have been proposed as influencing secondary sex rat...

Summary. In the ram, the size of the testes is related to the concentrations of FSH, LH and testosterone in the blood. A significant linear relationship is observed between testicular size and the level of FSH until a maximum after which no further increase in testis size is observed. Testicular size is also linearly related to the mean level (25 consecutive hours of sampling) of LH and testosterone in the blood, with apparently no upper limit, and to the freq...

In nuclear transfer reconstructed embryos, the co-ordination of donor nuclear and recipient cytoplasmic cell cycle phases is essential to maintain ploidy and prevent DNA damage. However, the stage of the cell cycle at the time of reconstruction and the method of reconstruction may also have a significant impact on the subsequent development of the embryo and fetus through a number of other mechanisms. This paper reviews some of the information currently available and proposes ...

For a follicle to reach dominance, in mono-ovulatory species such as cattle, requires the integration of a number of processes involving both extra-ovarian signals and intra-follicular paracrine and autocrine regulators. Ovarian transplant studies in both cattle and sheep demonstrated that it takes approximately 4 months for primordial follicles to reach dominance. Gonadotrophins are not a prerequisite for the continued growth of pre-antral follicles, unlike antral folliclcs, ...

Summary. In the wild pig mating activity is seasonal. The main breeding period is in late autumn but a second period may occur around April. It is known from other species that seasonal variations in breeding activity are mainly regulated by photoperiod.In the domestic pig seasonal influences on prolificacy still exist: for example, Al boars not only show decreased steroid synthesis, sperm counts and libido in summer compared with t...

Abstract unavailable© 1990 Journals of Reproduction & Fertility Ltd...

The inconsistency of data from experiments designed to show nutritional effects on embryonic survival is perplexing. However, a number of experimental models have provided some insight into the mechanisms that potentially mediate interactions between nutrition, metabolic state and embryonic survival. The developing ovarian follicle provides the maturational environment for the oocyte, and differences in follicular maturation are associated with differences in the ability ...