Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0016cpr13 | Embryonic and Placental Development | CPR2001

Gene expression during pre- and peri-implantation embryonic development in pigs

Maddox-Hyttel P. , Dinnyes A. , Laurincik J. , Rath D. , Niemann H. , Rosenkranz H. , Wilmut H.

Embryo technological procedures such as in vitro production and cloning by nuclear transfer are not as advanced in pigs as in cattle and cannot yet be applied under field conditions. The present paper focuses on genome activation in in vivo-derived, in vitro-produced and nuclear transfer pig embryos with special emphasis on the development of embryonic nucleoli, where the ribosomal RNA (rRNA) genes transcribed can be used as markers for genome ...

bp0017cpr7 | (1) | CPR2005

The growth hormone/prolactin gene family in ruminant placentae

Raeside J. I. , Christie H. L. , Renaud R. L. , Sinclair P. A.

A review of the remarkable production of steroids by the testes of the boar is presented, with the principal aims of highlighting the achievements of the Leydig cells and, at the same time, pointing to the considerable deficiencies in our understanding of its biological relevance. The onset of gonadal steroidogenesis at an early stage of sex differentiation and the pattern of pre- and postnatal secretion of steroids are outlined. This is followed by a list of steroids id...

bp0005rdr19 | Ovary-Uterus-Embryo Interactions | REDR2002

Regulation of embryo survival in cattle

Thatcher WW , Guzeloglu A , Meikle A , Kamimura S , Bilby T , Kowalski AA , Badinga L , Pershing R , Bartolome J , Santos JEP

Evidence is presented that bovine somatotrophin (bST) treatment of lactating dairy cows enhances both expression of oviductal insulin-like growth factor II (IGF-II) mRNA and endometrial insulin-like growth factor binding protein 3 (IGFBP-3) mRNA between day 3 and day 7 of the oestrous cycle. mRNA encoding growth hormone (GH) receptor in endometrial tissues increased between day 3 and day 7 of the oestrous cycle. The changes induced by bST treatment may contribute to stimulatio...

bp0005rdr32 | Ruminant Models for Human Clinical Medicine | REDR2002

Domestic ruminants as models for the elucidation of the mechanisms controlling ovarian follicle development in humans

Campbell BK , Souza C , Gong J , Webb R , Kendall N , Marsters P , Robinson G , Mitchell A , Telfer EE , Baird DT

It is necessary to understand the basic physiology underlying the complex process of folliculogenesis to address common causes of infertility and to devise innovative strategies to increase the efficiency of assisted reproduction technologies. Availability of suitable ovarian tissue is a major constraint to research in this area in humans, and monovulatory domestic ruminants represent a physiologically relevant model to elucidate basic mechanisms before more focused clinical i...

bp0010ised12 | (1) | ISED2019

Gliding into diapause: early embryo development in roe deer (Capreolus capreolus)

Drews B , Ulbrich SE , Rudolf Vegas A , Jewgenow K , Zahmel J , Roellig K , Ortmann S , Hildebrandt TB , Goeritz F

During mammalian diapause, the embryo is arrested at the blastocyst stage. In diapausing carnivores and marsupials, growth arrest of the blastocyst is coincident with a quiescent corpus luteum (CL). In the roe deer, progesterone is produced throughout diapause and the blastocyst exhibits very slow growth. To date, early roe deer embryos of known age have not been described. Therefore, it is not known at which stage and at what pace the roe deer embryo enters diapause. To colle...

bp0013cpr17 | Gamete Physiology | CPR1989

Expression and performance in transgenic pigs

Pursel V. G. , Bolt D. J. , Miller K. F. , Pinkert C. A. , Hammer R. E. , Palmiter R. D. , Brinster R. L.

Summary. Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called ‘transgenic’. The percentage of injected ova that developed into transgenic pigs varied among experiments from 0.31% to 1.73%. The percentage of transgenic pigs that expressed the fusion gene ranged from 17% to 100%.Eleven different regulatory s...