Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0017cpr11 | (1) | CPR2005

Inhibition of luteolysis and embryo-uterine interactions during the peri-implantation period in pigs

Ziecik A.J. , Blitek A. , Kaczmarek M.M. , Waclawik A. , Bogacki M.

Inhibition of luteolysis and establishment of pregnancy in pigs results from oestrogen secretion by the conceptuses and requires progesterone produced by the corpus luteum (CL). An integral part of maternal recognition of pregnancy in the pig is the redirection of prostaglandin (PG) F2α,secretion from endocrine (blood) to exocrine (uterus) direction and an increase of PGE2 synthesis in both the endometrium and conceptus. Uterine and conceptus ...

bp0007rdr31 | Unique Aspects of Reproduction in Diverse Ruminant Species | REDR2010

Assisted reproduction in Mediterranean wild ruminants: lessons from the Spanish ibex (Capra pyrenaica)

Santiago-Moreno J , Coloma MA , Toledano-Díaz A , Castaño C , Gómez-Brunet A , Lopez-Sebastián A

Summary. Despite apparent progress in reproductive technology as applied to wild ruminants, the success achieved in terms of the number of offspring that become healthy adults has remained low. Difficulties often arise through a lack of knowledge regarding appropriate cryopreservation techniques, and indeed through a lack of detailed information on the reproductive physiology of the species in question. The Spanish ibex (Capra pyrenaica) is a wild cap...

bp0005rdr9 | Reproductive Technology | REDR2002

Morphological assessment of preimplantation embryo quality in cattle

Maddox-Hyttel P , Gjørret JO , Vajta G , Alexopoulos NI , Lewis I , Trounson A , Viuff D , Laurincik J , Müller M , Tveden-Nyborg P , Thomsen PD

The extensive use of embryo technologies has emphasized the need for assessing embryo quality by morphological techniques, such as transmission electron microscopy, immunocytochemistry for confocal laser scanning microscopy and fluorescence in situ hybridization. By a combination of these techniques, it has been possible to demonstrate: (i) that rRNA gene activation, as monitored by embryonic nucleolar development, is comparable in bovine embryos developed in vivo...

bp0004rdr26 | Local Cellular and Tissue Communication | REDR1998

Roles of extracellular matrix in follicular development

Rodgers RJ , van Wezel IL , Irving-Rodgers HF , Lavranos TC , Irvine CM , Krupa M

The cellular biology and changes in the extracellular matrix of ovarian follicles during their development are reviewed. During growth of the bovine ovarian follicle the follicular basal lamina doubles 19 times in surface area. It changes in composition, having collagen IV α1–26 and laminin α1, ß2 and γl at the primordial stage, and collagen IV α1 and α2, reduced amounts of α3–α5, and a higher content of laminin α1, ß...

bp0005rdr10 | Reproductive Technology | REDR2002

The use of genomics and proteomics to understand oocyte and early embryo functions in farm animals

Sirard M-A , Dufort I , Coenen K , Tremblay K , Massicotte L , Robert C

Oocyte maturation, a simple and visible phenomenon, is about to be transformed into a complex and not so visible molecular cascade leading to the marking of the following generation. The study of oocyte maturation in mammals is progressively changing towards a more molecular approach. This review addresses the main challenges in the study of RNA extraction and quantification in oocytes and embryos as well as the importance of the mRNA maturation. The identification of specific...

bp0013cpr18 | Early Pregnancy | CPR1989

Causes and consequences of early embryonic diversity in pigs

Pope W. F. , Xie S. , Broermann D. M. , Nephew K. P.

Summary. Within 14 h of ovulation, follicular development in gilts was skewed towards a majority of mature follicles, based on their endocrine milieu. Oocyte maturation was also skewed, with a majority of the oocytes being meiotically more developed than the rest. Similarly, the pattern of ovulation in gilts was such that 70% of the follicles ovulated during a short period of time, while most of the remaining 30% ovulated over a more protracted period. Th...

bp0016cpr3 | Development of The Follicleand Corpus Luteum | CPR2001

Ovarian folliCular growth in sows*

Lucy M. C. , Liu J. , Boyd C. K. , Bracken C. J.

The resumption of ovarian follicular development during lactation and after weaning in sows is a complex process that ultimately determines rebreeding efficiency of sows. Ovarian follicular development before weaning is heterogeneous because multiple patterns of development are observed when individual sows are compared. Sows can have relatively inactive ovaries before weaning with follicles of < 2 mm in diameter. Other sows have nonovulatory follicular waves in which...

bp0018cpr3 | Physiological Roles of The Boar Ejaculate | CPR2009

Optimal characteristics of spermatozoa for semen technologies in pigs

Parrilla I. , Vazquez J.M. , Caballero I. , Gil M.A. , Hernandez M , Roca J. , Lucas X. , Martinez E.A.

Despite the great potential of sperm technologies such as sperm cryopreservation and sperm sex sorting for the improvement of different aspects of swine production, artificial insemination with fresh or stored semen is currently the only sperm technology used at a commercial scale in the pig industry. The lower reproductive performance associated with the use of these sperm technologies is the reason for such limited use. Since optimal characteristics are required for su...

bp0018cpr19 | Management of Ovarian Activity in Swine | CPR2009

Studies on fixed-time ovulation induction in the pig

Brussow K.P. , Schneider F. , Kanitz W. , Ratky J. , Kauffold J. , Wahner M.

A technology that allows for manipulating of oestrus and ovulation, and would then also allow for fixed-time insemination, can be of great benefit for swine farms that operate using sow batch management due, at least in part, to savings in labour and the production of large batches of evenly developed pigs. Thanks to the current knowledge on endocrine regulation of follicle development and ovulation, and the availability of numerous reproductively active substances such ...