Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0012cpr3 | Endocrinology of Follicular Development | CPR1985

Control of follicular development during and after lactation in sows

Britt J. H. , Armstrong J. D. , Cox Nancy M. , Esbenshade Nancy M.

Summary. Follicular development during early lactation in the sow is characterized by a large population of small-sized follicles and a small population of medium-sized follicles. As lactation progresses there is a gradual shift in number of follicles into medium- or large-sized categories and the percentage of follicles classified as atretic declines. Weaning at birth often leads to aberrant follicular development, apparently because the positive feedbac...

bp0016cpr22 | New Technologies | CPR2001

Deep intrauterine insemination and embryo transfer in pigs

Martinez E. A. , Vazquez J. M , Roca J. , Lucas X. , Gil M. A. , Vazquez J. L.

A new method for non-surgical deep intrauterine catheterization of pigs, without sedation of the sow, is described. Insemination results obtained with this method using fresh spermatozoa demonstrate that, in comparison to conventional artificial insemination (Al) (3 × 109 spermatozoa in 80-100 ml), a 20-60-fold reduction in the number of spermatozoa inseminated and at least a 8-10-fold reduction in the dose volume can be used without affecting fertility ...

bp0017cpr19 | (1) | CPR2005

Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination

Roca J. , Rodriguez-Martinez H. , Vazquez J.M. , Bolarin A. , Hernandez M. , Saravia F. , Wallgren M. , Martinez E.A.

Although cryopreservation of boar semen for artificial insemination (Al) was developed 35 years ago, cryopreservation conditions and Al strategies are still considered sub-optimal. Al with excessive numbers of frozenthawed sperm (5-6 x 109 cells), still does not achieve fertility levels similar to Al using liquid semen because of reduced sperm survival. Frozenthawed (FT) spermatozoa have therefore not been the preferred option for commercial breeding programme...

bp0003rdr10 | Development of the Reproductive Axis | REDR1994

Development of the sheep ovary during fetal and early neonatal life and the effect of fecundity genes

McNatty KP , Smith P , Hudson NL , Heath DA , Tisdall DJ , O W-S , Braw-Tal R

In female sheep fetuses, the mesonephros and genital ridge can be identified at days 20 and 23 of gestation (term = 145 days), respectively. Moreover oogonia can be observed at the genital ridge from as early as day 23. Around day 55 of gestation, some germ cells enter meiosis coincident with the arrival of mesonephric-derived somatic cells (i.e. the rete ovarii). From days 75, 100, 120 and 135 of gestation, primordial (one layer of flattened granulosa cells), primary (one...

bp0004rdr3 | Follicular Development | REDR1998

Molecular mechanisms regulating follicular recruitment and selection

Webb R , Campbell BK , Garverick HA , Gong JG , Gutierrez CG , Armstrong DG

Ovarian follicular growth and development is an integrated process encompassing both extraovarian signals, such as gonadotrophins and metabolic hormones, and intraovarian factors. Follicular development has been classified into gonadotrophin-independent and -dependent phases. In the latter, FSH provides the primary drive for follicular recruitment and LH is required for continued development of follicles to the preovulatory stage. A transient increase in circulating FSH preced...

bp0005rdr7 | Gamete-Somatic Cell Interactions | REDR2002

Mechanisms regulating follicular development and selection of the dominant follicle

Webb R , Nicholas B , Gong JG , Campbell BK , Gutierrez CG , Garverick HA , Armstrong DG

Reproductive function is an integrated process encompassing both extraovarian signals, such as gonadotrophins, and intrafollicular factors, such as locally produced growth factors. Initiation of primordial follicle growth and the early stages of folliculogenesis can occur without gonadotrophins. However, in vivo and in vitro studies indicate that FSH may stimulate the rate of preantral follicle growth and that it can take only 3 months for a primordial follic...

bp0006rdr2 | Male Reproduction | REDR2006

Seminal plasma effects on sperm handling and female fertility

Maxwell WMC , de#Graaf SP , Ghaoui R#El-Hajj , Evans G

The components of ruminant seminal plasma and their influence on the fertility of spermatozoa are reviewed. Seminal plasma can both inhibit and stimulate sperm function and fertility through the multifunctional actions of organic and inorganic components. These effects are now better understood because the composition of the seminal plasma, including its protein content and that of other structures, specifically membrane vesicles, has been clarified. Spermatozoa gain motility ...

bp0010ised9 | (1) | ISED2019

Lipid droplet utilization by the mouse embryo

Bisogno S , Arena R , Fic K , Gąsior Ł , Ptak GE

High numbers of lipid droplets (LDs) in mammalian eggs are stored and maintained throughout embryo development without marked signs of their utilization. It was previously demonstrated in large domestic mammals that removing lipids from the zygote does not influence post-implantation development in terms of the rate of delivered offspring. Previously studied pig and cow eggs contain considerable amounts of LDs, while mice have a very low level of ooplasmic lipids, which allows...