Bioscientifica Proceedings

Summary. Injection of prostaglandin (PG) F-2ct or its analogues has provided a . technique to induce parturition after Day 110 of gestation in the sow. The mean interval from PG injection to parturition ranges from 24 to 28 h, but only 50-60% of the sows farrow during an 8-10 h working day, and as many as 20% of sows may begin parturition before the injection of PG or > 22 h after the injection. The duration of parturition is positively associated with...

Keywords: pigs; nutrition; reproduction; condition; strategy© 1990 Journals of Reproduction & Fertility Ltd...

Cryopreservation advances capacitation-like changes in ram spermatozoa. These changes are reflected in an increased fertilizing ability compared with fresh spermatozoa, followed by an accelerated decline in fertilizing ability after incubation in vitro or in vivo. Furthermore, frozen–thawed spermatozoa are released earlier than fresh spermatozoa after binding to oviduct cells in vitro, confirming their physiological readiness to participate in f...

Summary. Early embryo mortality during the first two weeks of development is an important factor in the decreasing fertility of dairy cattle. During this period the embryo needs to correctly establish the first three lineages, namely epiblast, hypoblast and trophoblast so as to survive. The morphological and molecular events underlying these lineage decisions are discussed, drawing on information derived predominantly from mice and cattle.<p class="abstext...

In contrast to nuclear inheritance, cytoplasmic inheritance in mammals is derived mostly, if not exclusively, from the maternal line. Mitochondria, and their DNA molecules (mtDNA), are the genetic units of this method of inheritance. Mammalian mtDNA codes for 13 enzymes used in the mitochondrial energy-generating pathway, oxidative phosphorylation, 22 tRNAs and two rRNAs. Although all transcripts of mtDNA and their translational products remain in the mitochondria, most ...

Consistent production of large quantities of fertile semen is a primary concern of boar management programmes. Common visual tests such as motility are poor indicators of the fertilizing capacity of an ejaculate. Research studies have demonstrated that, in general, if motility is 60% or greater, there are no relationships among the percentage of motile spermatozoa, in vitro sperm penetration rates, farrowing rates and litter size. Biological and environmental fac...

Considerable progress has been made in the in vitro production of pig embryos using improved methods for in vitro maturation (IVM) and fertilization (IVF). Despite the progress, polyspermic penetration remains a problem for in vitro-matured oocytes. Variation among boars, ejaculates and IVF protocols used in different laboratories appears to influence the incidence of polyspermy. Recent studies indicate that oviduct cells and their secretions p...

Pigs begin behavioural preparations for birth about 1-2 days before parturition. Prepartum sows wander to select a suitable site and then construct a maternal nest. The signal that initiates this behavioural cascade probably results from fetal maturation but is unknown. However, endogenous PGF2a appears to be involved early on in an endocrine pathway that projects to the brain and can generate most of the prepartum behavioural components. This period of intense activity ...

There is still much we do not understand about the metabolic requirements of oocytes and embryos. Their remarkable metabolic plasticity during in vitro culture has hampered our ability to make significant advances in culture media design that would better support optimal physiology. A new tool, metabolomics, may revolutionize what we know about the interactions between embryos and their culture environment. Armed with this knowledge, we may design more effective culture ...

The physiological mechanisms controlling ovulation rate in mammals involve a complex exchange of endocrine signals between the pituitary gland and the ovary, and a localized exchange of intraovarian hormones between the oocyte and its adjacent somatic cells. The discoveries in sheep of mutations in bone morphogenetic protein 15 (BMP15) and bone morphogenetic protein receptor type IB (BMPR-IB) together with recent findings on the physiological effects of growth differentiation ...