Bioscientifica Proceedings

Abstract unavailable© 1982 The authors...

Summary. Microbes commonly infect the female genital tract of cattle, causing uterine disease, abortion, and infertility. In particular, postpartum uterine disease develops in about 40% of dairy cows following infections with Gram-negative Escherichia coli and Gram-positive bacteria, such as Trueperella pyogenes. These infections damage tissues and cause inflammation in the endometrium, and perturb follicle growth and function in the ovar...

The capacity of pig uterine tissues to recognize and respond to maternal and conceptus signals determines whether pregnancy can be established and defines the environment in which embryonic and fetal growth occur. Limits of uterine capacity may be defined genetically. However, the extent to which functional uterine capacity approaches genetic potential may be determined, in part, by the success of organizational events associated with growth, morphogenesis and cytodiffere...

A successful mating involves the coordination of many physiological events including insemination, gamete transport and ovulation. As more basic information about these processes becomes available, it is apparent that there are opportunities for manipulating these physiological events and enhancing the reproductive success of natural and artificial matings. The primary intent of this review is to examine the effectiveness of several of these strategies, as determined by t...

Cryopreservation advances capacitation-like changes in ram spermatozoa. These changes are reflected in an increased fertilizing ability compared with fresh spermatozoa, followed by an accelerated decline in fertilizing ability after incubation in vitro or in vivo. Furthermore, frozen–thawed spermatozoa are released earlier than fresh spermatozoa after binding to oviduct cells in vitro, confirming their physiological readiness to participate in f...

Summary. Studies have consistently demonstrated that 4-cell pig embryos can be cultured to the blastocyst stage in a simple salt solution containing bovine serum albumin (BSA). Pig embryos appear to be detrimentally affected by lower levels of lactate and pyruvate than are mouse embryos, but in general their in-vitro requirements are similar. Results from embryos cultured between the 4-cell and blastocyst stages are consistent enough to allow the use of c...

In contrast to nuclear inheritance, cytoplasmic inheritance in mammals is derived mostly, if not exclusively, from the maternal line. Mitochondria, and their DNA molecules (mtDNA), are the genetic units of this method of inheritance. Mammalian mtDNA codes for 13 enzymes used in the mitochondrial energy-generating pathway, oxidative phosphorylation, 22 tRNAs and two rRNAs. Although all transcripts of mtDNA and their translational products remain in the mitochondria, most ...

Consistent production of large quantities of fertile semen is a primary concern of boar management programmes. Common visual tests such as motility are poor indicators of the fertilizing capacity of an ejaculate. Research studies have demonstrated that, in general, if motility is 60% or greater, there are no relationships among the percentage of motile spermatozoa, in vitro sperm penetration rates, farrowing rates and litter size. Biological and environmental fac...

Considerable progress has been made in the in vitro production of pig embryos using improved methods for in vitro maturation (IVM) and fertilization (IVF). Despite the progress, polyspermic penetration remains a problem for in vitro-matured oocytes. Variation among boars, ejaculates and IVF protocols used in different laboratories appears to influence the incidence of polyspermy. Recent studies indicate that oviduct cells and their secretions p...

Pigs begin behavioural preparations for birth about 1-2 days before parturition. Prepartum sows wander to select a suitable site and then construct a maternal nest. The signal that initiates this behavioural cascade probably results from fetal maturation but is unknown. However, endogenous PGF2a appears to be involved early on in an endocrine pathway that projects to the brain and can generate most of the prepartum behavioural components. This period of intense activity ...