Bioscientifica Proceedings

Summary. Our understanding of the synthesis and production of follicular steroids and prostaglandins (PG) in the pig is based largely on in-vitro studies with granulosa and theca interna tissues obtained from Graafian follicles at various stages of maturation. As the follicle enlarges before the LH surge, granulosa cells exhibit a decrease in FSH receptors and are less responsive to FSH in terms of cAMP production. Concurrently, there is an increase in gr...

Summary. Within 14 h of ovulation, follicular development in gilts was skewed towards a majority of mature follicles, based on their endocrine milieu. Oocyte maturation was also skewed, with a majority of the oocytes being meiotically more developed than the rest. Similarly, the pattern of ovulation in gilts was such that 70% of the follicles ovulated during a short period of time, while most of the remaining 30% ovulated over a more protracted period. Th...

The capacity of pig uterine tissues to recognize and respond to maternal and conceptus signals determines whether pregnancy can be established and defines the environment in which embryonic and fetal growth occur. Limits of uterine capacity may be defined genetically. However, the extent to which functional uterine capacity approaches genetic potential may be determined, in part, by the success of organizational events associated with growth, morphogenesis and cytodiffere...

The development of new technologies that would increase the efficiency for generation of transgenic livestock and would overcome some of the problems associated with random insertion of the transgene will greatly benefit animal agriculture. A potential alternative technology to pronuclear injection for the generation of transgenic pigs involves the isolation, culture and genetic manipulation of cell lines that can be reintroduced into the embryo for participation in the f...

The resumption of ovarian follicular development during lactation and after weaning in sows is a complex process that ultimately determines rebreeding efficiency of sows. Ovarian follicular development before weaning is heterogeneous because multiple patterns of development are observed when individual sows are compared. Sows can have relatively inactive ovaries before weaning with follicles of < 2 mm in diameter. Other sows have nonovulatory follicular waves in which...

An overview is presented on the structure and function of the pig oviduct in relation to sperm capacitation and oocyte development in vivo. In pigs, a functional sperm reservoir is established in the uterotubal junction-isthmus when sperm deposition occurs before ovulation. Capacitation is assumed to occur in this location, and spermatozoa progress towards the ampullaryisthmic junction at about the time of ovulation as a consequence of capacitation and hyperacti...

In pigs, as in other species, fetal growth retardation is associated with reduced birth weight and increased risk of fetal and neonatal death. As there are few opportunities after birth to remedy the detrimental effects of low birth weight, it is important to understand both the intrinsic and extrinsic factors associated with inadequate fetal growth and to determine when growth retarded fetuses deviate from the growth trajectory of their normal sized littermates. Inadequately ...

Functional genomics is an experimental approach that incorporates genomewide or system-wide experimentation, expanding the scope of biological investigation from studying single genes to studying potentially all genes at once in a systematic manner. This technology is highly appealing because of its high throughput and relatively low cost. Furthermore, analysis of gene expression using microarrays is likely to be more biologically relevant than the conventional paradigm ...

Abstract unavailable© 2009 Society for Reproduction and Fertility...

Next generation DNA sequencing is a high throughput method of sequencing DNA samples in parallel. During the last 10 years, this technology has expanded to include sequencing and quantification of an entire transcriptome. The advantage of this method of transcriptome analysis is that it allows the investigator to detect previously unknown genes and splice variants as well as detect potential DNA polymorphisms. Application of this technology, especially when used to perfo...