Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0001redr10 | (1) | REDR1980

Endocrine patterns associated with puberty in male and female cattle

Schams D , Schallenberger E , Gombe S , Karg H

Summary. In four studies secretion patterns of LH, FSH, prolactin, testosterone and progesterone were measured in male and female cattle to determine endocrine changes associated with sexual maturation. Two periods of increasing gonadotrophin secretion were observed, the second one coinciding with puberty. A short luteal phase of 8–12 days precedes the first oestrus at 10–11 or 14 months of age. The testosterone values of the bulls increased with age...

bp0007rdr22 | The Ruminant Corpus Luteum | REDR2010

Regulation of corpus luteum development and maintenance: specific roles of angiogenesis and action of prostaglandin F

Miyamoto A , Shirasuna K , Shimizu T , Bollwein H , Schams D

Development of the corpus luteum (CL) in ruminants occurs in a rapid and time-dependent manner within 1 week after ovulation, with morphologic and biochemical changes in the cells of the theca interna and granulosa cells of the preovulatory follicle. These changes involve luteinisation of steroidogenic cells and angiogenesis to establish normal luteal function (progesterone secretion). The CL is composed of a large number of vascular endothelial cells, large and small steroido...

bp0012cpr2 | Endocrinology of Follicular Development | CPR1985

Maturation of ovarian follicles in the prepubertalgilt

Christenson R. K. , Ford J. J. , Redmer D. A.

Summary. The processes of follicle development and puberty are closely related, and both are associated with maturation of the hypothalamic—pituitary—ovarian axis. Prenatal development of the ovary is independent of gonadotrophic stimulation. Beyond 60 days of age (postnatally), tertiary follicles develop and gonadotrophins begin to influence ovarian follicular development. Negative feedback regulation of pituitary gonadotrophins by ovarian secretions dev...

bp0014cpr1 | Regulation of Oocyte and Embryonic Development in Pigs | CPR1993

Follicle–oocyte–sperminteractions in vivo and in vitro in pigs

Sirard M. A. , Dubuc A. , Bolamba D. , Zheng Y. , Coenen K.

In vitro culture has provided new information on the mechanisms involved in fertilization where two completely different cells fuse together. At the same time, results obtained in vitro have led to new questions. Does the follicle influence the final maturation process of the oocyte and does the oviduct regulate the normal behaviour of spermatozoa? Recent studies indicate a critical influence of both the follicular compartment and the oviduct on the norm...

bp0013cpr17 | Gamete Physiology | CPR1989

Expression and performance in transgenic pigs

Pursel V. G. , Bolt D. J. , Miller K. F. , Pinkert C. A. , Hammer R. E. , Palmiter R. D. , Brinster R. L.

Summary. Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called ‘transgenic’. The percentage of injected ova that developed into transgenic pigs varied among experiments from 0.31% to 1.73%. The percentage of transgenic pigs that expressed the fusion gene ranged from 17% to 100%.Eleven different regulatory s...

bp0004rdr28 | Local Cellular and Tissue Communication | REDR1998

Growth factors and extracellular matrix proteins in interactions of cumulus–oocyte complex, spermatozoa and oviduct

Einspanier R , Gabler C , Bieser B , Einspanier A , Berisha B , Kosmann M , Wollenhaupt K , Schams D

The expression and localization of selected growth factor systems and extracellular matrix (ECM) components that may influence oocyte maturation and fertilization within the mammalian oviduct are reported. Fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) systems could be detected by use of RT–PCR, RNase protection assay (RPA) and immunohistochemistry in bovine follicles, bovine cumulus–oocyte complexes (COC) and bovine and marmoset oviduct...

bp0006rdr22 | Embryo Gene Expression | REDR2006

Gene expression analysis of single preimplantation bovine embryos and the consequence for developmental potential

Ruddock-D'Cruz NT , Hall VJ , Tecirlioglu RT , French AJ

Preimplantation embryo development typically involves sequential morphological events connecting embryonic cleavage, morula compaction and blastocyst formation, and occurs in parallel with transcriptional regulation, specifically, the maternal to embryonic transition. The underlying homeostatic and metabolic mechanisms governing embryo development are influenced by both genetic and epigenetic factors that respond to environmental stimuli and may impact development during later...