Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0003rdr22 | Regulation of Gonadotrophin Secretion | REDR1994

Dopaminergic control of LH secretion by the Al5 nucleus in anoestrous ewes

Thiery JC , Gayrard V , Le Corre S , Viguié C , Martin GB , Chemineau P , Malpaux B

Annual variations in the secretion of LH are responsible for seasonal changes in ovulatory activity in ewes. This hormonal pattern reflects an increase in the intensity of the negative feedback exerted by oestradiol under long days. Neuro-pharmacological studies have shown that this inhibition of LH secretion involves activation of catecholaminergic systems from preoptic and mediobasal hypothalamus (MBH) by oestradiol during anoestrus, and that 5-hydroxytryptamine inputs may a...

bp0010ised9 | (1) | ISED2019

Lipid droplet utilization by the mouse embryo

Bisogno S , Arena R , Fic K , Gąsior Ł , Ptak GE

High numbers of lipid droplets (LDs) in mammalian eggs are stored and maintained throughout embryo development without marked signs of their utilization. It was previously demonstrated in large domestic mammals that removing lipids from the zygote does not influence post-implantation development in terms of the rate of delivered offspring. Previously studied pig and cow eggs contain considerable amounts of LDs, while mice have a very low level of ooplasmic lipids, which allows...

bp0013cpr18 | Early Pregnancy | CPR1989

Causes and consequences of early embryonic diversity in pigs

Pope W. F. , Xie S. , Broermann D. M. , Nephew K. P.

Summary. Within 14 h of ovulation, follicular development in gilts was skewed towards a majority of mature follicles, based on their endocrine milieu. Oocyte maturation was also skewed, with a majority of the oocytes being meiotically more developed than the rest. Similarly, the pattern of ovulation in gilts was such that 70% of the follicles ovulated during a short period of time, while most of the remaining 30% ovulated over a more protracted period. Th...

bp0018cpr28 | Control of Prenatal Development | CPR2009

Prenatal programming of postnatal development in the pig

Foxcroft G.R. , Dixon W.T. , Dyck M.K. , Novak S. , Harding J.C.S. , Almeida F.C.R.L.

Studies of low birth weight offspring have a long history in pig science. These pigs have reduced growth potential and poor carcass quality compared to their higher birth weight littermates. In contemporary commercial sows with between 10 and 15 total pigs born/litter, between-litter differences in average birth weight appear to make the largest contribution to variation in postnatal growth performance, independent of numbers born. Low birth weight is a characteristic of...

bp0018cpr30 | Control of Prenatal Development | CPR2009

Functional genomic approaches for the study of fetal/placental development in swine with special emphasis on imprinted genes

Bischoff S.R. , Tsai S. , Hardison N. , Motsinger-Reift A.A. , Freking B.A. , Piedrahita J.A.

This chapter describes the application of functional genomic approaches to the study of imprinted genes in swine. While there are varied definitions of "functional genomics", in general they focus on the application of DNA microarrays, single nucleotide polymorphism (SNP) arrays, and other high coverage genomic analyses, and their combination with downstream methods of gene modification such as silencing RNA (siRNA) and viral and non-viral transfection. Between the initi...

bp0019cpr4 | Gametes and Embryos | CPR2013

Cryopreservation of female germplasm in pigs

Tam�s Somfai1, Kazuhiro Kikuchi2, Hiroyuki Kaneko2, Junko Noguchi2, , Koji Yoshioka3, Takashi Nagai1

Cryopreservation of female germplasm has basic importance in preservation and distribution of genetic lines in farm animals. Although vitrification technology has been applied for the cryopreservation of porcine oocytes and ovarian tissues, reduced developmental competence of preserved oocytes and the lack of offspring produced from them underlines the importance for further developments in cryopreservation protocols for this purpose. This review discusses the problems o...