Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0010ised13 | (1) | ISED2019

Trophectoderm and embryoblast proliferate at slow pace in the course of embryonic diapause in the roe deer (Capreolus capreolus)

Rüegg AB , Bernal S , Moser FN , Rutzen I , Ulbrich SE

The European roe deer (Capreolus capreolus) was the first mammal in which embryonic diapause has been described. While diapause is characterized by a complete developmental arrest in some species, roe deer blastocysts show a very slow, yet continuous growth. To date, it is neither known whether this growth is accompanied by developmental progression nor whether it is uniform in both, the trophectoderm (TE) and the inner cell mass (ICM). We collected roe deer blastocysts during...

bp0013cpr18 | Early Pregnancy | CPR1989

Causes and consequences of early embryonic diversity in pigs

Pope W. F. , Xie S. , Broermann D. M. , Nephew K. P.

Summary. Within 14 h of ovulation, follicular development in gilts was skewed towards a majority of mature follicles, based on their endocrine milieu. Oocyte maturation was also skewed, with a majority of the oocytes being meiotically more developed than the rest. Similarly, the pattern of ovulation in gilts was such that 70% of the follicles ovulated during a short period of time, while most of the remaining 30% ovulated over a more protracted period. Th...

bp0018cpr28 | Control of Prenatal Development | CPR2009

Prenatal programming of postnatal development in the pig

Foxcroft G.R. , Dixon W.T. , Dyck M.K. , Novak S. , Harding J.C.S. , Almeida F.C.R.L.

Studies of low birth weight offspring have a long history in pig science. These pigs have reduced growth potential and poor carcass quality compared to their higher birth weight littermates. In contemporary commercial sows with between 10 and 15 total pigs born/litter, between-litter differences in average birth weight appear to make the largest contribution to variation in postnatal growth performance, independent of numbers born. Low birth weight is a characteristic of...

bp0018cpr30 | Control of Prenatal Development | CPR2009

Functional genomic approaches for the study of fetal/placental development in swine with special emphasis on imprinted genes

Bischoff S.R. , Tsai S. , Hardison N. , Motsinger-Reift A.A. , Freking B.A. , Piedrahita J.A.

This chapter describes the application of functional genomic approaches to the study of imprinted genes in swine. While there are varied definitions of "functional genomics", in general they focus on the application of DNA microarrays, single nucleotide polymorphism (SNP) arrays, and other high coverage genomic analyses, and their combination with downstream methods of gene modification such as silencing RNA (siRNA) and viral and non-viral transfection. Between the initi...

bp0019cpr4 | Gametes and Embryos | CPR2013

Cryopreservation of female germplasm in pigs

Tam�s Somfai1, Kazuhiro Kikuchi2, Hiroyuki Kaneko2, Junko Noguchi2, , Koji Yoshioka3, Takashi Nagai1

Cryopreservation of female germplasm has basic importance in preservation and distribution of genetic lines in farm animals. Although vitrification technology has been applied for the cryopreservation of porcine oocytes and ovarian tissues, reduced developmental competence of preserved oocytes and the lack of offspring produced from them underlines the importance for further developments in cryopreservation protocols for this purpose. This review discusses the problems o...

bp0005rdr19 | Ovary-Uterus-Embryo Interactions | REDR2002

Regulation of embryo survival in cattle

Thatcher WW , Guzeloglu A , Meikle A , Kamimura S , Bilby T , Kowalski AA , Badinga L , Pershing R , Bartolome J , Santos JEP

Evidence is presented that bovine somatotrophin (bST) treatment of lactating dairy cows enhances both expression of oviductal insulin-like growth factor II (IGF-II) mRNA and endometrial insulin-like growth factor binding protein 3 (IGFBP-3) mRNA between day 3 and day 7 of the oestrous cycle. mRNA encoding growth hormone (GH) receptor in endometrial tissues increased between day 3 and day 7 of the oestrous cycle. The changes induced by bST treatment may contribute to stimulatio...