Bioscientifica Proceedings

Summary. Synthesis of biologically active LH is complex, due in part to its hetero-dimeric subunit structure and to the numerous post-translation modifications of each subunit. Through the use of mammalian expression vectors we have been able to introduce the bovine α subunit and LH-β genes into a Chinese hamster ovary cell line deficient in dihydrofolate reductase. The bovine genes are actively expressed and the Chinese hamster ovary cells secrete b...

Summary. Single genes can now be added routinely to the genome of mice by molecular manipulation as simple Mendelian dominants; this complements the normal process of reproduction to give ‘transgenic’ animals. Success in ruminants is limited to a few examples in sheep and although gene expression has yet to be documented, there is every reason to expect that it will be achieved. The application of this technology to livestock improvement depends on...

Summary. Several manipulation techniques including nuclear injections, nuclear transplantation, embryo splitting, chimaera production and sperm injection are discussed with special reference to their application in the pig. The nuclear injection technique is likely to be of greatest use for gene transfer. Gene transfer is feasible for pig embryos. but it is very inefficient. Efficiencies of various steps in the successful production of transgenic offsprin...

Keywords: pig; models; metabolism© 1990 Journals of Reproduction & Fertility Ltd...

Keywords: counter current transfer; ovary; oviduct; uterus; pig© 1990 Journals of Reproduction & Fertility Ltd...

Summary. Behavioural responses can have direct effects on reproduction when the performance of the behaviour contributes to productivity (e.g. achievement of copulation). Alternatively, there are indirect effects where a behavioural change is insufficient to allow adaptation to an environmental change and so the animal has to resort to physiological mechanisms with potential adverse effects on productivity.Boar contact has substantial ...

The ancestral wild pig is a short day length seasonal breeder. The domestic pig appears to have retained some of this seasonality as evidenced by a reduction in fertility during the summer—autumn period. The most important aspect of this seasonality is a reduction in the number of mated sows that farrow. Many of these sows conceive and embryos develop normally for 20 - 25 days before pregnancy is terminated and the sow returns to oestrus (25 - 35 days after mating). In ot...

Efficient production of high quality semen is of major importance to artificial insemination (Al) organizations. The semen produced should be free of contagious organisms, be of high quality, have good storage properties, fertilizing capacity and be of high genetic value. The best approach to prevent the spreading of microorganisms via semen in the process of Al is to collect semen from boars free from specific diseases, for example pseudorabies virus or leptospirosis. An...

Research in France and in the UK confirms the prolificacy of the Chinese Meishan breed to be about three to four piglets greater than that of control Large White females. Crossbreeding studies clearly indicate that this breed difference is due to genes acting in the dam and not in the litter itself. There is high heterosis for litter size in F1 Meishan x Large White crossbred females, such that their litter size is similar to or greater than that of purebred Me...

The immune system discriminates 'self' from 'non-self', and eliminates that which it determines to be non-self. Mammalian pregnancy appears to represent a failure of self-non-self discrimination, yet it is a highly successful reproductive strategy. We present evidence that the immune system of the female pig responds to the challenges of both mating and the presence of conceptuses. Mating induces an influx of inflammatory leukocytes into the endometrial stroma and uterine...