Bioscientifica Proceedings

Next generation DNA sequencing is a high throughput method of sequencing DNA samples in parallel. During the last 10 years, this technology has expanded to include sequencing and quantification of an entire transcriptome. The advantage of this method of transcriptome analysis is that it allows the investigator to detect previously unknown genes and splice variants as well as detect potential DNA polymorphisms. Application of this technology, especially when used to perfo...

It is necessary to understand the basic physiology underlying the complex process of folliculogenesis to address common causes of infertility and to devise innovative strategies to increase the efficiency of assisted reproduction technologies. Availability of suitable ovarian tissue is a major constraint to research in this area in humans, and monovulatory domestic ruminants represent a physiologically relevant model to elucidate basic mechanisms before more focused clinical i...

Abstract unavailable© 2009 Society for Reproduction and Fertility...

Evidence is presented that bovine somatotrophin (bST) treatment of lactating dairy cows enhances both expression of oviductal insulin-like growth factor II (IGF-II) mRNA and endometrial insulin-like growth factor binding protein 3 (IGFBP-3) mRNA between day 3 and day 7 of the oestrous cycle. mRNA encoding growth hormone (GH) receptor in endometrial tissues increased between day 3 and day 7 of the oestrous cycle. The changes induced by bST treatment may contribute to stimulatio...

Summary. Recent research clearly shows that fusion genes can be microinjected into a pronucleus of an ovum and integrate into the pig genome. Animals with such fusion genes are called ‘transgenic’. The percentage of injected ova that developed into transgenic pigs varied among experiments from 0.31% to 1.73%. The percentage of transgenic pigs that expressed the fusion gene ranged from 17% to 100%.Eleven different regulatory s...

Summary. Despite apparent progress in reproductive technology as applied to wild ruminants, the success achieved in terms of the number of offspring that become healthy adults has remained low. Difficulties often arise through a lack of knowledge regarding appropriate cryopreservation techniques, and indeed through a lack of detailed information on the reproductive physiology of the species in question. The Spanish ibex (Capra pyrenaica) is a wild cap...

It has been demonstrated that variations in litter size or ovulation rate in different breeds of sheep can be associated with the segregation of several major genes. This set of natural mutants constitutes a valuable resource to determine key points in the biochemical pathways controlling the development of ovarian follicles. The French genetic programmes were devised to identify two of these genes: the Booroola (FecB) and Lacaune genes. The FecB pro...

Summary. Reproductive traits can range from lowly to moderately heritable. Genomic technologies provide a powerful tool for improving selection for traits that are lowly heritable, sex-linked, or not expressed until later in life. Therefore, as genomic technologies become a part of selection decisions, there is a critical need to understand how specific gene variants affect reproductive traits in cattle. Both classical quantitative genetics approaches and geno...

Conceptus development in mammals depends on an intra-uterine environment filled with histotroph that includes molecules that are secreted by uterine epithelia and/or selectively transported into the uterine lumen. In pigs, total recoverable glucose, fructose, arginine, leucine and glutamine increase in histotroph with advancing days of the peri-implantation period of pregnancy and in allantoic fluid later in gestation. During pregnancy, the uterine luminal epithelium (LE...

Currently, there is no method to diagnose pregnancy non-invasively in most wildlife species that experience delayed implantation and pseudopregnancy, either during embryonic diapause or placental pregnancy. The aim of this study was to utilize farm-raised mink (Neovison vison) as a model species to evaluate changes in the fecal proteome associated with pregnancy. Specific objectives were to: 1) determine if fecal peptides were differentially abundant in parturient ver...