Searchable, peer-reviewed, open-access proceedings from bioscience and biomedical conferences

bp0019cpr3 | Gametes and Embryos | CPR2013

Boar seminal plasma proteins and their relevance to reproductive technologies

W.L. Flowers1, K.R. Stewart1, T. Gall2, S. Novak3, M.K. Dyck3 , and R.N. Kirkwood4 , Nagase H , Woolley DE

Seminal plasma proteins participate in a number of events important for fertilization and the establishment of pregnancy. As a result, attempts have been made to use them to enhance reproductive performance associated with several swine reproductive technologies. Inclusion of seminal plasma into cryopreservation and sex-sorting protocols improved sperm viability and membrane integrity and suppressed capacitation-like changes which are considered to be major challenges as...

bp0015cpr17 | Advances in Biotechnology in Pig Reproduction | CPR1997

Developments in transgenictechniques in pigs

Nottle M. B. , Nagashima H. , Verma P. J. , Du Z. T. , Grupen Z. T. , Ashman Z. T. , Macilfatrick S.

Manipulation of the pig genome is currently restricted to the random insertion of new DNA using pronuclear microinjection. This method suffers from a number of inherent limitations, the majority of which result from the inability to control the site at which the transgene becomes integrated. These drawbacks, together with the need to be able to target existing genes, will result in the replacement of pronuclear injection by new methods that have the capability to direct i...

bp0016cpr14 | Embryonic and Placental Development | CPR2001

Functional analysis of autocrine and paracrine signalling at the uterine–conceptus interface in pigs

Jaeger L. A. , Johnson L. A. , Ka H. , Gadow J. G. , Burghardt R. C. , Spencer T. E. , Bazer F.W.

The complexity of implantation necessitates intimate dialogue between conceptus and maternal cells, and precise coordination of maternal and conceptus signalling events. Maternal and conceptus-derived steroid hormones, growth factors and cytokines, as well as integrins and their ligands, have important and inter-related roles in mediating adhesion between apical aspects of conceptus trophectoderm and maternal uterine luminal epithelium that leads to formation of an epithelioch...

bp0004rdr28 | Local Cellular and Tissue Communication | REDR1998

Growth factors and extracellular matrix proteins in interactions of cumulus–oocyte complex, spermatozoa and oviduct

Einspanier R , Gabler C , Bieser B , Einspanier A , Berisha B , Kosmann M , Wollenhaupt K , Schams D

The expression and localization of selected growth factor systems and extracellular matrix (ECM) components that may influence oocyte maturation and fertilization within the mammalian oviduct are reported. Fibroblast growth factor (FGF) and vascular endothelial growth factor (VEGF) systems could be detected by use of RT–PCR, RNase protection assay (RPA) and immunohistochemistry in bovine follicles, bovine cumulus–oocyte complexes (COC) and bovine and marmoset oviduct...

bp0006rdr22 | Embryo Gene Expression | REDR2006

Gene expression analysis of single preimplantation bovine embryos and the consequence for developmental potential

Ruddock-D'Cruz NT , Hall VJ , Tecirlioglu RT , French AJ

Preimplantation embryo development typically involves sequential morphological events connecting embryonic cleavage, morula compaction and blastocyst formation, and occurs in parallel with transcriptional regulation, specifically, the maternal to embryonic transition. The underlying homeostatic and metabolic mechanisms governing embryo development are influenced by both genetic and epigenetic factors that respond to environmental stimuli and may impact development during later...

bp0013cpr21 | Early Pregnancy | CPR1989

Embryonicsteroids and the establishment of pregnancy in pigs

Geisert R. D. , Zavyt M. T. , Moffattt R. J. , Blair R. M. , Yellin T.

Summary. In the pig, establishment of pregnancy begins about 11-12 days after the start of oestrus. The ability of pig conceptuses to synthesize and release oestrogens during this period, as well as the ability of exogenous oestrogens to induce pseudopregnancy when administered from Day 11-15 of the oestrous cycle, provide evidence for an involvement of oestrogen in the maternal recognition of pregnancy in the sow. Oestrogen derived from the conceptus or ...

bp0008rdr16 | Challenges in Optimization of Reproductive Performance | REDR2014

Endocrine disruptors and ovine reproductive development

Lea Richard G , Byers Andrew S , Bellingham Michelle , Cotinot Corinne , Evans Neil , Mandon-Pepin Beatrice , Sinclair Kevin D , Fowler Paul A

Summary. Declining fertility and perturbations in reproductive development in a variety of species have been linked to exposure to endocrine disrupting chemicals (EDCs), which are ubiquitous in the environment. Ruminants are largely exposed to such chemicals in sewage sludge fertiliser widely used in animal production systems. This has been investigated experimentally through the deliberate exposure of pregnant ewes to sewage sludge fertilised pastures or cont...